Predict and visualize the effect of every possible mutation within an enhancer sequence on TF binding. For mutations within predicted sites in the wild-type sequence, the mutation can either have no effect, decrease, optimize or ablate the TF-DNA interactions as quantified by either PWM-binding score or affinity reference dataset. For mutations residing outside predicted sites in the wild-type sequence, the mutation can either have no effect or create a de novo binding site, whose strength of interaction is again quantified either PWM-binding score or affinity reference dataset. If a mutation has multiple effects on TF binding, all effects are reported. When available, use of a binding site core and reference affinity dataset are always preferred over PWM approaches, as PWM-binding scores are worse predictors of binding affinity.

Links: Tutorial Video Documentation